In this study, fermentable sugars and cellulose nanocrystals (CNCs) were co-produced from endoglucanase treatment of wood pulp, followed by acid hydrolysis. Enzymatic hydrolysis was performed using two endoglucanases differentiated by the presence or absence of a cellulose-binding domain (CBD). The enzyme with an intact CBD gave the higher glucan conversion (up to 14.1 ± 1.2 wt %) and improved the degree of crystallinity of the recovered wood pulp fiber (up to 83.0 ± 1.0%). Thus, this endoglucanase-assisted treatment successfully removed amorphous content from the original cellulosic feedstock. CNC recovery (16.9 ± 0.7 wt %) from the feedstock going into the acid hydrolysis was improved relative to untreated pulp (13.2 ± 0.6 wt %). The mass loss from enzymatic treatment did not cause a decrease in the CNC yield from the starting material. The characteristics of CNCs obtained through acid hydrolysis (with or without enzyme treatment of pulp) were analyzed using X-ray diffraction, transmission electron microscopy, dynamic light scattering, Fourier transform infrared spectroscopy, and differential scanning calorimetry as characterization techniques. The CNCs generated through acid hydrolysis of endoglucanase-treated wood pulp displayed comparable properties relative to those generated using untreated pulp. Thus, endoglucanase treatment can enable co-production of CNCs and sugars for biofuel fermentation.
Keywords: acid hydrolysis; cellulose nanocrystals; degree of crystallinity; endoglucanase treatment; fermentable sugars; wood pulp.