Conformational restraint as a strategy for navigating towards lysosomes

Chem Commun (Camb). 2018 Oct 23;54(85):12010-12013. doi: 10.1039/c8cc06155f.

Abstract

Using the conformational restraint strategy, we developed a hydrazonate-derived coumarin into a lysosome targeting probe for imaging native formaldehyde at the subcellular level. Using this probe, we observed the overproduction of formaldehyde in lysosomes when cells were treated with endoplasmic reticulum (ER) stress inducers, suggesting the involvement of formaldehyde in protein misfolding.

MeSH terms

  • Cell Line
  • Coumarins / chemical synthesis
  • Coumarins / chemistry*
  • Coumarins / toxicity
  • Endoplasmic Reticulum Stress
  • Fluorescence
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • Fluorescent Dyes / toxicity
  • Formaldehyde / analysis
  • Formaldehyde / metabolism*
  • Humans
  • Hydrazones / chemical synthesis
  • Hydrazones / chemistry*
  • Hydrazones / toxicity
  • Limit of Detection
  • Lysosomes / metabolism*
  • Microscopy, Confocal / methods
  • Microscopy, Fluorescence / methods
  • Molecular Conformation
  • Protein Folding / drug effects

Substances

  • Coumarins
  • Fluorescent Dyes
  • Hydrazones
  • Formaldehyde