Single-Channel Single-Molecule Detection (SC-SMD) System

Methods Mol Biol. 2018:1843:189-201. doi: 10.1007/978-1-4939-8704-7_16.

Abstract

The combination of single-molecule fluorescence imaging and electrophysiology provides a powerful tool to explore the stoichiometry and functional properties of ionic channels, simultaneously. Here, we describe a typical SC-SMD experiment from the preparation of plasmids containing the genes encoding for the channels of interest fused to fluorescent proteins to the use of the SC-SMD system for simultaneous patch clamping and single-molecule determinations.

Keywords: Patch clamp; Single channel; Single molecule; TRPC channels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electrophysiological Phenomena*
  • Gene Expression
  • HEK293 Cells
  • Humans
  • Ion Channel Gating*
  • Ion Channels / genetics
  • Ion Channels / metabolism*
  • Patch-Clamp Techniques* / instrumentation
  • Patch-Clamp Techniques* / methods
  • Single Molecule Imaging* / methods
  • Transfection

Substances

  • Ion Channels