Vitamin B12 protects against DNA damage induced by hydrochlorothiazide

Karem H Alzoubi; Erva Bayraktar; Omar Khabour; Sayer I Al-Azzam

doi:10.1016/j.jsps.2018.04.005

Vitamin B12 protects against DNA damage induced by hydrochlorothiazide

Saudi Pharm J. 2018 Sep;26(6):786-789. doi: 10.1016/j.jsps.2018.04.005. Epub 2018 Apr 3.

Authors

Karem H Alzoubi¹, Erva Bayraktar¹, Omar Khabour², Sayer I Al-Azzam¹

Affiliations

¹ Department of Clinical Pharmacy, Jordan University of Science and Technology, Irbid, Jordan.
² Department of Medical Laboratory Sciences, Jordan University of Science and Technology, Irbid, Jordan.

Abstract

DNA damage induced by hydrochlorothiazide was previously reported in cultured human lymphocytes. In this study, we aimed to investigate the harmful effects of hydrochlorothiazide on DNA by measuring the spontaneous frequency of sister chromatid exchanges (SCEs) in cultured human lymphocytes. We also aimed to investigate the possible protection of that damage by vitamin B12. The results showed that hydrochlorothiazide (5 µg/mL) significantly increased the frequency of sister chromatid exchanges (P < 0.001) in human lymphocytes in comparison with control. Additionally, the frequency of hydrochlorothiazide-induced SCEs was significantly decreased by co-treatment with vitamin B12 at concentration of 13.5 µg/mL (P < 0.001). In conclusion, hydrochlorothiazide is genotoxic to human lymphocytes and its toxicity is reduced by vitamin B12.

Keywords: DNA damage; Genotoxicity; Hydrochlorothiazide; Sister chromatids exchange; Vitamin B12.