The TORC1-Nem1/Spo7-Pah1/lipin axis regulates microautophagy induction in budding yeast

Muhammad Arifur Rahman; Mashu Terasawa; Md Golam Mostofa; Takashi Ushimaru

doi:10.1016/j.bbrc.2018.09.011

The TORC1-Nem1/Spo7-Pah1/lipin axis regulates microautophagy induction in budding yeast

Biochem Biophys Res Commun. 2018 Oct 2;504(2):505-512. doi: 10.1016/j.bbrc.2018.09.011. Epub 2018 Sep 7.

Authors

Muhammad Arifur Rahman¹, Mashu Terasawa², Md Golam Mostofa¹, Takashi Ushimaru³

Affiliations

¹ Graduate School of Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8021, Japan.
² Department of Science, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8021, Japan.
³ Graduate School of Science and Technology, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8021, Japan; Department of Science, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8021, Japan. Electronic address: ushimaru.takashi@shizuoka.ac.jp.

PMID: 30201264
DOI: 10.1016/j.bbrc.2018.09.011

Abstract

Nutrient starvation and inactivation of target of rapamycin complex 1 (TORC1) protein kinase promotes macroautophagy. Macroautophagy is a lipid-consuming process, and Nem1/Spo7 protein phosphatase and Pah1/lipin phosphatidate phosphatase are activated after TORC1 inactivation, supporting macroautophagy induction in the budding yeast Saccharomyces cerevisiae. On the other hand, whether and how microautophagy, which also consumes lipids, is regulated by TORC1 is controversial. Here we show that TORC1 inactivation induces microautophagy in budding yeast. Vps27, but not Atg1, Atg7, or Atg8, was required for TORC1 inactivation-induced microautophagy. Furthermore, the Nem1/Spo7-Pah1 axis was also critical for microautophagy induction. Thus, the TORC1-Nem1/Spo7-Pah1 axis is a master regulator of not only macroautophagy but also microautophagy in budding yeast.

Keywords: Lipin; Microautophagy; Nem1; Pah1; Rapamycin; Target of rapamycin complex 1 (TORC1).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Autophagy*
Endosomal Sorting Complexes Required for Transport / metabolism
Lipid Metabolism
Lipids / chemistry
Membrane Proteins / metabolism*
Nuclear Proteins / metabolism*
Phosphatidate Phosphatase / metabolism*
Saccharomyces cerevisiae / metabolism*
Saccharomyces cerevisiae Proteins / metabolism*
Saccharomycetales
Sirolimus / chemistry
Transcription Factors / metabolism*

Substances

Endosomal Sorting Complexes Required for Transport
Lipids
Membrane Proteins
Nem1 protein, S cerevisiae
Nuclear Proteins
SPO7 protein, S cerevisiae
Saccharomyces cerevisiae Proteins
TORC1 protein complex, S cerevisiae
Transcription Factors
VPS27 protein, S cerevisiae
PAH1 protein, S cerevisiae
Phosphatidate Phosphatase
Sirolimus