Abstract
The application of phosphorodiamidate technology to pyrimidine and purine nucleosides with anticancer activity to potentially overcome the resistance mechanisms associated with parent nucleosides is reported. Sixteen symmetrical phosphorodiamidates were prepared from the natural amino acids l-alanine and glycine. All the compounds were evaluated for their cytotoxic activity against a wide panel of solid and leukaemic tumour cell lines. In addition, a carboxypeptidase Y assay was performed on a representative phosphorodiamidate in order to reveal the putative bioactivation pathway for the reported phosphorodiamidate-type prodrugs.
Keywords:
ProTide; antitumour agents; nucleoside analogues; phosphate prodrugs; phosphorodiamidates.
© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antineoplastic Agents / chemical synthesis
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Antineoplastic Agents / chemistry
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Antineoplastic Agents / pharmacology*
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Cathepsin A / chemistry
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Cell Line, Tumor
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Enzyme Assays
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Humans
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Mice
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Molecular Structure
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Organophosphorus Compounds / chemical synthesis
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Organophosphorus Compounds / chemistry
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Organophosphorus Compounds / pharmacology*
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Prodrugs / chemical synthesis
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Prodrugs / chemistry
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Prodrugs / pharmacology*
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Purine Nucleosides / chemical synthesis
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Purine Nucleosides / chemistry
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Purine Nucleosides / pharmacology*
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Pyrimidine Nucleosides / chemical synthesis
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Pyrimidine Nucleosides / chemistry
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Pyrimidine Nucleosides / pharmacology*
Substances
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Antineoplastic Agents
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Organophosphorus Compounds
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Prodrugs
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Purine Nucleosides
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Pyrimidine Nucleosides
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Cathepsin A