Study of the Involvement of Phosphatidic Acid Formation in the Expression of Wound-Responsive Genes in Cotton

Lipids. 2018 Jun;53(6):589-599. doi: 10.1002/lipd.12058. Epub 2018 Sep 10.

Abstract

Plants use phospholipase D (PLD, EC 3.1.4.4)/phosphatidic acid (PtdOH) for the transduction of environmental signals including those coming from wounding. Based on our previous findings suggesting that wound-induced PLDα-derived PtdOH can act as a local signaling molecule in cotton (Gossypium hirsutum), we show that wounding immediately increases local NADPH oxidase (NADPHox) and cellulose synthase A (CeSA) gene expression. After developing a novel fluorimetric assay for the investigation of n-butanol inhibitory effect on PLD activity, we show that only NADPHox gene upregulation is reduced when n-butanol is applied prior to wounding. This suggests that NADPHox is a possible downstream target of PLD function, while a different CeSA-involving response system may exist in cotton. Overall, this study provides new knowledge on signal-transduction mechanisms following wounding of cotton leaves.

Keywords: Butanol; Cellulose synthase; Cotton (Gossypium hirsutum); Fluorimetric PLD assay; NADPH oxidase; Wounding.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Gene Expression Regulation, Plant / genetics*
  • Glucosyltransferases / genetics*
  • Glucosyltransferases / metabolism
  • Gossypium / genetics*
  • Gossypium / metabolism
  • NADPH Oxidases / genetics*
  • NADPH Oxidases / metabolism
  • Phosphatidic Acids / biosynthesis*
  • Phosphatidic Acids / chemistry
  • Plant Leaves / genetics
  • Plant Leaves / metabolism
  • Signal Transduction / genetics

Substances

  • Phosphatidic Acids
  • NADPH Oxidases
  • Glucosyltransferases
  • cellulose synthase