Defining Lineage-Specific Membrane Fluidity Signatures that Regulate Adhesion Kinetics

Takahisa Matsuzaki; Shinya Matsumoto; Toshiharu Kasai; Emi Yoshizawa; Satoshi Okamoto; Hiroshi Y Yoshikawa; Hideki Taniguchi; Takanori Takebe

doi:10.1016/j.stemcr.2018.08.010

Defining Lineage-Specific Membrane Fluidity Signatures that Regulate Adhesion Kinetics

Stem Cell Reports. 2018 Oct 9;11(4):852-860. doi: 10.1016/j.stemcr.2018.08.010. Epub 2018 Sep 6.

Authors

Takahisa Matsuzaki¹, Shinya Matsumoto², Toshiharu Kasai², Emi Yoshizawa², Satoshi Okamoto², Hiroshi Y Yoshikawa³, Hideki Taniguchi⁴, Takanori Takebe⁵

Affiliations

¹ Institute of Research, Tokyo Medical and Dental University (TMDU), 15-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan; Department of Regenerative Medicine, Yokohama City University Graduate School of Medicine, Kanazawa-ku 3-9, Yokohama, Kanagawa 236-0004, Japan.
² Department of Regenerative Medicine, Yokohama City University Graduate School of Medicine, Kanazawa-ku 3-9, Yokohama, Kanagawa 236-0004, Japan.
³ Department of Chemistry, Saitama University, Shimo-okubo 255, Sakura-ku, Saitama 338-8570, Japan.
⁴ Department of Regenerative Medicine, Yokohama City University Graduate School of Medicine, Kanazawa-ku 3-9, Yokohama, Kanagawa 236-0004, Japan; Advanced Medical Research Center, Yokohama City University, Kanazawa-ku 3-9, Yokohama, Kanagawa 236-0004, Japan.
⁵ Institute of Research, Tokyo Medical and Dental University (TMDU), 15-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan; Department of Regenerative Medicine, Yokohama City University Graduate School of Medicine, Kanazawa-ku 3-9, Yokohama, Kanagawa 236-0004, Japan; Advanced Medical Research Center, Yokohama City University, Kanazawa-ku 3-9, Yokohama, Kanagawa 236-0004, Japan; Division of Gastroenterology, Hepatology & Nutrition, Developmental Biology, Center for Stem Cell and Organoid Medicine (CuSTOM), Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229-3039, USA; Department of Pediatrics, University of Cincinnati College of Medicine, 3333 Burnet Avenue, Cincinnati, OH 45229-3039, USA. Electronic address: takanori.takebe@cchmc.org.

Abstract

Cellular membrane fluidity is a critical modulator of cell adhesion and migration, prompting us to define the systematic landscape of lineage-specific cellular fluidity throughout differentiation. Here, we have unveiled membrane fluidity landscapes in various lineages ranging from human pluripotency to differentiated progeny: (1) membrane rigidification precedes the exit from pluripotency, (2) membrane composition modulates activin signaling transmission, and (3) signatures are relatively germ layer specific presumably due to unique lipid compositions. By modulating variable lineage-specific fluidity, we developed a label-free "adhesion sorting (AdSort)" method with simple cultural manipulation, effectively eliminating pluripotent stem cells and purifying target population as a result of the over 1,150 of screened conditions combining compounds and matrices. These results underscore the important role of tunable membrane fluidity in influencing stem cell maintenance and differentiation that can be translated into lineage-specific cell purification strategy.

Keywords: cell adhesion; cell sorting; fluidic modulator; membrane fluidity; pluripotency.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Adhesion
Cell Differentiation
Cell Lineage*
Cell Membrane / metabolism
Humans
Induced Pluripotent Stem Cells / cytology
Kinetics
Membrane Fluidity*

Grants and funding

P30 DK078392/DK/NIDDK NIH HHS/United States