Selection and Use of Intracellular Antibodies

Sandrine Moutel; Clément Nizak; Franck Perez

doi:10.1007/978-1-4939-8648-4_25

Selection and Use of Intracellular Antibodies

Methods Mol Biol. 2018:1827:491-503. doi: 10.1007/978-1-4939-8648-4_25.

Authors

Sandrine Moutel^{1

2}, Clément Nizak³, Franck Perez^{4

5}

Affiliations

¹ CNRS, UMR144, Paris, France.
² Institut Curie, PSL Research University, Paris, France.
³ Laboratoire de Biochimie, ESPCI Paris, PSL Research University, CNRS UMR8231 Chimie Biologie Innovation, Paris, France.
⁴ CNRS, UMR144, Paris, France. Franck.Perez@curie.fr.
⁵ Institut Curie, PSL Research University, Paris, France. Franck.Perez@curie.fr.

PMID: 30196513
DOI: 10.1007/978-1-4939-8648-4_25

Abstract

Intracellularly expressed recombinant antibodies, or intrabodies, are powerful tools for cell biology studies as well as therapeutic applications. Cell biologists use them to either block the intracellular antibody target or to image endogenous target dynamics. We describe here methods to select recombinant antibodies from antibody phage display libraries and to subsequently express them as fluorescent intrabodies.

Keywords: Intrabody; Live cell imaging; Phage display.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies / isolation & purification*
Fluorescent Antibody Technique
Humans
Imaging, Three-Dimensional
Intracellular Space / metabolism*
Protein Engineering / methods*

Substances

Antibodies