Porcine CD83 is a glycosylated dimeric protein existing naturally in membrane-bound and soluble forms

Shanshan Huo; Jianlou Zhang; Fengyang Wu; Yuzhu Zuo; Dan Cui; Xiujin Li; Zhenyu Zhong; Fei Zhong

doi:10.1016/j.dci.2018.09.002

Porcine CD83 is a glycosylated dimeric protein existing naturally in membrane-bound and soluble forms

Dev Comp Immunol. 2019 Jan:90:60-69. doi: 10.1016/j.dci.2018.09.002. Epub 2018 Sep 5.

Authors

Shanshan Huo¹, Jianlou Zhang¹, Fengyang Wu², Yuzhu Zuo¹, Dan Cui¹, Xiujin Li³, Zhenyu Zhong⁴, Fei Zhong⁵

Affiliations

¹ Laboratory of Molecular Virology and Immunology, College of Animal Science and Technology/College of Veterinary Medicine, Agricultural University of Hebei, Baoding, Hebei, 071000, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding, Hebei, 071000, China.
² Laboratory of Molecular Virology and Immunology, College of Animal Science and Technology/College of Veterinary Medicine, Agricultural University of Hebei, Baoding, Hebei, 071000, China.
³ Department of Biotechnology, College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao, Hebei, 066004, China.
⁴ Department of Immunology, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA. Electronic address: zhenyu.zhong@utsouthwestern.edu.
⁵ Laboratory of Molecular Virology and Immunology, College of Animal Science and Technology/College of Veterinary Medicine, Agricultural University of Hebei, Baoding, Hebei, 071000, China; Hebei Engineering and Technology Research Center of Veterinary Biotechnology, Baoding, Hebei, 071000, China. Electronic address: zhongf@hebau.edu.cn.

PMID: 30193829
DOI: 10.1016/j.dci.2018.09.002

Abstract

Human and mouse CD83 have been well characteized, however, the other mammalian CD83 genes have not been cloned and characterized. In this study, the porcine CD83 (pCD83) was cloned, expressed and characterized, and showed that the pCD83 gene has 81% and 74% homologies with humans and mice, respectively, which was identified to be glycosylated when expressed in eukaryotic cells, existing naturally in two forms: membrance-bound CD83 (mCD83) and soluble CD83 (sCD83), the latter was identified to be generated mainly from mCD83 by proteolytic shedding. The pCD83 was a dimmer mediated by intermolecular disulfide bond formed by the fifth cysteine in the exrtracellular domain. Functionally, the recombinant porcine sCD83 was preliminarily tested to have the ability to inhibit DC-mediated T cell activition. This study provided necessary fundation for further investigation on pCD83 functions.

Keywords: CD83; Characterization; Cloning; Porcine; Soluble CD83.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antigens, CD / genetics*
Antigens, CD / metabolism
CD83 Antigen
Cells, Cultured
Cloning, Molecular
Dendritic Cells / immunology*
Dimerization
Humans
Immunoglobulins / genetics*
Immunoglobulins / metabolism
Lymphocyte Activation
Membrane Glycoproteins / genetics*
Membrane Glycoproteins / metabolism
Mice
Sequence Alignment
Swine / immunology*
T-Lymphocytes / immunology*

Substances

Antigens, CD
Immunoglobulins
Membrane Glycoproteins