Transcriptomic analysis of the oviduct of pigs during the peri-conceptional period

Abstract

The optimal environment in the oviduct is created by adjusting its ultrastructure and secretory capacity to protect gametes and embryos. It was hypothesized that direct contact between the isthmic epithelium and 2- and 4-cell-stage embryos would alter the transcriptomic profile of the isthmus in pigs. Microarray analysis was performed to determine the alterations in gene expression of the isthmus on Days 2-3 of pregnancy in pigs (after natural mating) during embryo presence in the oviduct. Of 43,803 microarray probes, 354 (0.81%) transcripts were altered (P-value ≤ 0.05 and fold-change ≥ 1.2) on the days of pregnancy when assessments were made. Of these 354 transcripts, 118 (33.3%) were up-regulated, and 236 (66.7%) were down-regulated. A total of 57 (48.3%) up-regulated and 73 down-regulated (30.9%) transcripts were classified into gene ontology categories. Of the 354 altered genes, 36 (10.2%) were categorized into the Toll-like or NOD-like receptor signaling pathway, in the immune system subcategory. Selected genes engaged in maternal immune function were down-regulated. The up-regulated genes were involved in epigenetic regulation, the protection of embryos against oxidative stress and xenobiotics and the control of estrogen metabolism. The 2- and 4-cell-stage embryos might, therefore, affect the oviductal transcriptome to optimize the intra-oviductal milieu, which is necessary to support proper development of embryos. The results of this study indicates the pig oviduct has the capacity to alter its transcriptomic profile as a result of early embryo development after natural mating.

Keywords: Embryos; Isthmus; Microarray analysis; Peri-conceptional period.