13C-NMR-based metabolic fingerprinting of Citrus-type crude drugs

Takashi Tsujimoto; Taichi Yoshitomi; Takuro Maruyama; Yutaka Yamamoto; Takashi Hakamatsuka; Nahoko Uchiyama

doi:10.1016/j.jpba.2018.08.044

¹³C-NMR-based metabolic fingerprinting of Citrus-type crude drugs

J Pharm Biomed Anal. 2018 Nov 30:161:305-312. doi: 10.1016/j.jpba.2018.08.044. Epub 2018 Aug 28.

Authors

Takashi Tsujimoto¹, Taichi Yoshitomi¹, Takuro Maruyama¹, Yutaka Yamamoto², Takashi Hakamatsuka¹, Nahoko Uchiyama³

Affiliations

¹ Division of Pharmacognosy, Phytochemistry and Narcotics, National Institute of Health Sciences, 3-25-26, Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-9501, Japan.
² Tochimoto Tenkaido Co., Ltd., Oniya Kaibara-cho, Tamba, Hyogo 669-3315, Japan.
³ Division of Pharmacognosy, Phytochemistry and Narcotics, National Institute of Health Sciences, 3-25-26, Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-9501, Japan. Electronic address: nuchiyama@nihs.go.jp.

PMID: 30193199
DOI: 10.1016/j.jpba.2018.08.044

Abstract

Five Citrus-type crude drugs (40 samples) were classified using ¹³C-NMR spectra-based metabolomics. The following eight metabolites were identified from the loading plots of multivariate analysis of the ¹³C-NMR spectra; naringin, neohesperidin, narirutin, synephrine, sucrose, α-glucose, β-glucose, and limonene. ¹³C-NMR spectra-based metabolic fingerprinting is a promising strategy for classifying crude drugs.

Keywords: Citrus; Crude drugs; Metabolomics; Multivariate analysis; NMR.

MeSH terms

Carbon-13 Magnetic Resonance Spectroscopy / methods*
Citrus / chemistry*
Molecular Structure
Phytochemicals / chemistry*
Phytochemicals / metabolism*

Substances

Phytochemicals