A photoenzymatic NADH regeneration system was established. The combination of deazariboflavin as a photocatalyst with putidaredoxin reductase enabled the selective reduction of NAD+ into the enzyme-active 1,4-NADH to promote an alcohol dehydrogenase catalysed stereospecific reduction reaction. The catalytic turnover of all the reaction components was demonstrated. Factors influencing the efficiency of the overall system were identified.
Keywords: biocatalysis; enantioselectivity; ketones; photochemistry; reduction.
© 2018 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.