Joint action of theophylline and guanosine diphosphate leads to the additional release of Ca2+ from intracellular stores of oocytes with intact microfilaments and microtubules, additional release of Ca2+ was not observed in oocytes after the joint action of prolactin and guanosine triphosphate. In the presence of an inhibitor of polymerization microfilaments cytochalasin D additional release of Ca2+ not detected in oocytes treated by theophylline and guanosine diphosphate, but treatment of oocyte by prolactin with guanosine triphosphate leads to the additional release of Ca2+ from intracellular stores. Nocodazole had no effect on the release of Ca2+ from intracellular stores of oocytes treated by theophylline with guanosine diphosphate or prolactin with guanosine triphosphate. The joint action of prolactin and guanosine triphosphate on devitrified oocytes with destroyed microfilaments (as a result of defrosting) leads to additional Ca2+ exit from the intracellular stores. The obtained data expand ideas concerning features of regulation of intracellular processes in porcine oocytes.