Biological effect of hydrothermally synthesized silica nanoparticles within crystalline hydroxyapatite coatings for titanium implants

Abstract

Development of functional coatings for artificial bone implants that strengthen the osseointegration and accelerate bone healing processes is urgently needed in the biomedical field. In this study we present biological effect of novel composite coatings with different concentration of silica nanoparticles within crystalline hydroxyapatite matrix (HAp-SiO₂) synthesized on titanium under hydrothermal conditions. Samples were analyzed for their elemental composition, structure, bioactivity and in vitro cytotoxicity. The results indicate the formation and homogeneous distribution of silica nanoparticles on the surface of hexagonal hydroxyapatite (HAp) crystals. The coatings show improved bioactivity in comparison with pure HAp after 4 days of immersion in simulated body fluid (SBF). The responses of human osteoblast-like cells (MG-63) cultured onto the synthesized materials provide evidence that HAp-SiO₂ composites exhibit good biocompatibility. We propose that this is because HAp-SiO₂ composites favor biomineralization process with cell proliferation remaining unaffected, regardless of the amount of silica. Furthermore, SEM and fluorescence measurements demonstrate that HAp-SiO₂ had positive effect on cell morphology, favoring cell adhesion.

Keywords: Bioactive coatings; Hydrothermal synthesis; Hydroxyapatite; Silica nanoparticles.