Relationship between antibody titres against human cytomegalovirus detected by enzyme immunoassay and titres to individual viral polypeptides studied by immunoblotting

Microbiologica. 1986 Jul;9(3):373-9.

Abstract

Several sera with different IgG and IgM titres measured by enzyme immunoassay were studied in serial dilutions for their reactivity with human Cytomegalovirus structural polypeptides separated in SDS-PAGE and electrotransferred to nitrocellulose. The different immunogenicity of the viral proteins is documented and a hypothetical evolution of the humoral immune response to individual polypeptides is presented.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Viral / analysis*
  • Cytomegalovirus / immunology*
  • Cytomegalovirus Infections / immunology*
  • Electrophoresis, Polyacrylamide Gel
  • Female
  • Humans
  • Immunoenzyme Techniques
  • Immunoglobulin G / analysis
  • Immunoglobulin M / analysis
  • Immunologic Techniques
  • Kinetics
  • Peptides / immunology
  • Pregnancy
  • Pregnancy Complications, Infectious / immunology
  • Viral Proteins / immunology*

Substances

  • Antibodies, Viral
  • Immunoglobulin G
  • Immunoglobulin M
  • Peptides
  • Viral Proteins