Effects of ultrasound irradiation on the characterization and bioactivities of the polysaccharide from blackcurrant fruits

Abstract

In this study, the influence of ultrasound irradiation on the characterization and bioactivities of the polysaccharide from blackcurrant fruits (BCP, molecular weight: M_w = 3.26 × 10⁴ kDa) was investigated. Two degraded polysaccharides (U-400, M_w = 1.89 × 10⁴ kDa, and U-600, M_w = 1.32 × 10⁴ kDa) were obtained by different ultrasound powers of 400 W and 600 W, respectively. Compared with BCP, U-400 and U-600 showed 63.52% and 68.85% reductions in the particle size (Z_avg), respectively; moreover, the dynamic viscosity of BCP was reduced by 27.88%, and 33.63%, separately. The reducing sugar content and thermal stability increased with the increase of ultrasound intensity. The degraded polysaccharides contained the same monosaccharide species as those of BCP but at different molar ratios. Fourier transform infrared (FT-IR) and nuclear magnetic resonance (NMR) spectroscopic analysis confirmed that the degraded polysaccharides and BCP exhibited the similar structural features, which were mainly composed of six glycosidic bonds. A reduction in surface area of the flake-like structure was observed in the degraded polysaccharides compared to that of BCP, and they had no triple helix structure. Furthermore, the precise structural characteristics of U-600 were identified by 2D NMR analysis. The results of the bioactivity assays indicated that the ultrasound irradiation could evidently enhance the antioxidant (hydroxyl and superoxide radicals scavenging, lipid peroxidation inhibition, and DNA damage protection activities), α-amylase and α-glucosidase inhibition activities of BCP. These activities increased in the order of U-600 > U-400 > BCP. In particular, the DNA protection and α-amylase inhibition activities for U-600 were 52.19 ± 1.34% and 75.98 ± 0.77%, respectively, which were 2 times higher than those of BCP. U-600 prepared with the higher-intensity ultrasound exhibited the best physicochemical properties and bioactivities among the three polysaccharides. These results suggested that ultrasound irradiation was an efficient, green method to produce value-added polysaccharide for use in functional food or medicine.

Keywords: Acarbose (PubChem CID: 41774); Antioxidant activity; Ascorbic acid (PubChem CID: 54670067); Blackcurrant polysaccharides; Characterization; Congo red (PubChem CID: 11313); D-Galacturonic acid (PubChem CID: 439215); Dextran (PubChem CID: 4125253); Trichloroacetic acid (PubChem CID: 6421); Ultrasound irradiation; p-Nitrophenyl-α-D-glucopyranoside (PubChem CID: 259380); α-Amylase inhibition; α-Glucosidase inhibition.