Reply to Kang and Brooks: Comment on the interpretation of binding of Pra1, the fungal immune evasion protein from Candida albicans to the human C3 and on the conformational changes of C3 upon activation: Kang and Brooks Optimization of biolayer-interferometry-based binding assay of he interaction between the Candida albicans protein Pra1 and complement protein C3
1 Department of Infection Biology, Leibniz-Institute for Natural Product Research and Infection Biology, Hans-Knöll-Institute, Jena, Germany; Center for Sepsis Control and Care, University Hospital Jena, Germany.
2 Department of Infection Biology, Leibniz-Institute for Natural Product Research and Infection Biology, Hans-Knöll-Institute, Jena, Germany.
3 Department of Infection Biology, Leibniz-Institute for Natural Product Research and Infection Biology, Hans-Knöll-Institute, Jena, Germany; Microbial Immunology Group, Leibniz-Institute for Natural Product Research and Infection Biology, Hans-Knöll-Institute, Jena, Germany.
4 University of Würzburg, Institute for Virology and Immunobiology, Würzburg, Germany.
5 Friedrich-Schiller-University, Jena, Germany.
6 Institute of Medical Microbiology, Medical School Hannover, Germany.