XLSY: Extra-Large NMR Spectroscopy

Yulia Pustovalova; Maxim Mayzel; Vladislav Yu Orekhov

doi:10.1002/anie.201806144

XLSY: Extra-Large NMR Spectroscopy

Angew Chem Int Ed Engl. 2018 Oct 22;57(43):14043-14045. doi: 10.1002/anie.201806144. Epub 2018 Oct 1.

Authors

Yulia Pustovalova¹, Maxim Mayzel², Vladislav Yu Orekhov^{1

2}

Affiliations

¹ Department of Chemistry and Molecular Biology, University of Gothenburg, P.O. Box 465, Gothenburg, 405 30, Sweden.
² Swedish NMR Centre, University of Gothenburg, P.O. Box 465, Gothenburg, 405 30, Sweden.

Abstract

NMR studies of intrinsically disordered proteins and other complex biomolecular systems require spectra with the highest resolution and dimensionality. An efficient approach, extra-large NMR spectroscopy, is presented for experimental data collection, reconstruction, and handling of very large NMR spectra by a combination of the radial and non-uniform sampling, a new processing algorithm, and rigorous statistical validation. We demonstrate the first high-quality reconstruction of a full seven-dimensional HNCOCACONH and two five-dimensional HACACONH and HN(CA)CONH experiments for a representative intrinsically disordered protein α-synuclein. XLSY will significantly enhance the NMR toolbox in challenging biomolecular studies.

Keywords: NMR spectroscopy; XLSY; intrinsically disordered protein; non-uniform sampling.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Algorithms
Intrinsically Disordered Proteins / chemistry
Nuclear Magnetic Resonance, Biomolecular / methods*
Protein Conformation
alpha-Synuclein / chemistry

Substances

Intrinsically Disordered Proteins
alpha-Synuclein