Detection of choline and hydrogen peroxide in infant formula milk powder with near infrared upconverting luminescent nanoparticles

Ling Zhang; Shuhan Yin; Jiaze Hou; Wenping Zhang; Hui Huang; Yongxin Li; Cong Yu

doi:10.1016/j.foodchem.2018.07.128

Detection of choline and hydrogen peroxide in infant formula milk powder with near infrared upconverting luminescent nanoparticles

Food Chem. 2019 Jan 1:270:415-419. doi: 10.1016/j.foodchem.2018.07.128. Epub 2018 Jul 19.

Authors

Ling Zhang¹, Shuhan Yin², Jiaze Hou², Wenping Zhang³, Hui Huang⁴, Yongxin Li⁵, Cong Yu⁶

Affiliations

¹ College of Food Science and Engineering, Jilin University, Changchun 130025, PR China.
² College of Food Science and Engineering, Jilin University, Changchun 130025, PR China; State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
³ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China.
⁴ College of Food Science and Engineering, Jilin University, Changchun 130025, PR China. Electronic address: huanghui@jlu.edu.cn.
⁵ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China. Electronic address: liyongxin@ciac.ac.cn.
⁶ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China. Electronic address: congyu@ciac.ac.cn.

PMID: 30174065
DOI: 10.1016/j.foodchem.2018.07.128

Abstract

Choline is an essential nutrient for the growth and development of the baby, and therefore it is often added to infant formula. In this paper, a novel sensor for choline determination in infant formula is developed based on upconverting nanoparticles (UCNPs) with near infrared luminescence. UCNPs-based detection can avoid the interference of background fluorescence from complex samples, and thus provide high selectivity and sensitivity. It was observed that in the presence of Fe³⁺, polyacrylic acid coated UCNPs were quenched to 3% of its original intensity. The degree of quenching was among the best for UCNPs. Hydrogen peroxide could oxidize Fe²⁺ to Fe³⁺, which caused quenching of the upconversion luminescence. A new H₂O₂ detection method was thus established. In addition, choline could be hydrolyzed to betaine by choline oxidase, and at the same time produced H₂O₂, which also caused luminescence quenching through Fe²⁺ oxidation. Therefore, selective choline sensing was achieved.

Keywords: Choline detection; Fe(3+) detection; H(2)O(2) detection; Luminescence; Upconverting nanoparticles.

MeSH terms

Choline / chemistry*
Choline / isolation & purification
Humans
Hydrogen Peroxide / chemistry*
Hydrogen Peroxide / isolation & purification
Infant
Infant Formula / chemistry*
Infant, Newborn
Nanoparticles / chemistry

Substances

Hydrogen Peroxide
Choline