Layer-by-layer chitosan-decorated pristine graphene on screen-printed electrodes by one-step electrodeposition for non-enzymatic hydrogen peroxide sensor

Qin Zhu; Bo Liang; Yu Cai; Qingpeng Cao; Tingting Tu; Bobo Huang; Lu Fang; Xuesong Ye

doi:10.1016/j.talanta.2018.07.038

Layer-by-layer chitosan-decorated pristine graphene on screen-printed electrodes by one-step electrodeposition for non-enzymatic hydrogen peroxide sensor

Talanta. 2018 Dec 1:190:70-77. doi: 10.1016/j.talanta.2018.07.038. Epub 2018 Jul 27.

Authors

Qin Zhu¹, Bo Liang², Yu Cai¹, Qingpeng Cao¹, Tingting Tu¹, Bobo Huang¹, Lu Fang³, Xuesong Ye⁴

Affiliations

¹ Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering and Instrument Science, Innovation Center for Minimally Invasive Technique and Device, Zhejiang University, Hangzhou 310027, PR China.
² Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering and Instrument Science, Innovation Center for Minimally Invasive Technique and Device, Zhejiang University, Hangzhou 310027, PR China. Electronic address: boliang1986@zju.edu.cn.
³ College of Life Information Science and Instrument Engineering, Hangzhou Dianzi University, Hangzhou 310018, PR China.
⁴ Biosensor National Special Laboratory, Key Laboratory of Biomedical Engineering of Ministry of Education, College of Biomedical Engineering and Instrument Science, Innovation Center for Minimally Invasive Technique and Device, Zhejiang University, Hangzhou 310027, PR China. Electronic address: yexuesong@zju.edu.cn.

PMID: 30172543
DOI: 10.1016/j.talanta.2018.07.038

Abstract

Layer-by-layer chitosan-decorated pristine graphene on screen-printed electrodes was achieved by one-step electrodeposition method and an enzyme-free hydrogen peroxide electrochemical biosensor was fabricated for its application. Negatively charged pristine graphene absorbed with positively charged chitosan by electrostatic interactions was electrodeposited on the electrodes. The successful immobilization of pristine graphene was confirmed by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), Raman spectroscopy, X-ray photoelectron spectroscopy (XPS) and electrochemical characterizations. The graphene-chitosan volume ratio and cyclic voltammetry scan cycles during the electrodeposition process were optimized. The resulting hydrogen peroxide biosensors showed a 178 times improved sensitivity and exhibited two wide linear detection ranges from 20 μM to 20 mM and from 20 mM to 60 mM. The biosensors also showed a good reproducibility, stability, selectivity and could be used in real samples detection. The superior electrochemical performance of graphene-chitosan was attributed to the preservation of excellent properties of pristine graphene, and the formation of layer-by-layer structure with high surface area. The proposed strategy of direct immobilization of pristine graphene can be extended for the immobilization of other nanomaterials and biomolecules.

Keywords: Chitosan; Co-electrodeposition; Hydrogen peroxide biosensor; Pristine graphene; Screen-printed electrodes.