Aptamer-based sensor for quantitative detection of mercury (II) ions by attenuated total reflection surface enhanced infrared absorption spectroscopy

Abstract

A sensing platform based on the attenuated total reflection surface enhanced infrared absorption spectroscopy (ATR-SEIRAS) technique and immobilized aptamer has been proposed herein for the selective detection of mercury ions (Hg²⁺). In the proposed platform, 5' thiolated 32-mer DNA probes with methylene blue at the 3' end were immobilized on a thin gold (Au) surface layer. Following Hg²⁺ ions interacting with T bases of the aptamer, T-Hg-T bonds are formed; resulting in a hairpin-shaped formation of the DNA and a detectable change in the IR absorbance of the sensing interface. Notably, the background noise produced by external molecules (e.g., water, non-specific binding molecules and bulk solution) is reduced to a negligible level by means of the ATR detection mode. It is shown that the proposed sensor has a linear response (R² = 0.986) with high sensitivity and good selectivity over the Hg²⁺ range of 0.01 μM-50 μM.

Keywords: Aptamer; Mercury ion; Microfluidics; The attenuated total reflection surface-enhanced infrared absorption spectroscopy.