Bacillus thuringiensis produces the lipopeptide thumolycin to antagonize microbes and nematodes

Dehong Zheng; Zhiyong Zeng; Bingbing Xue; Yaoyao Deng; Ming Sun; Ya-Jie Tang; Lifang Ruan

doi:10.1016/j.micres.2018.06.004

Bacillus thuringiensis produces the lipopeptide thumolycin to antagonize microbes and nematodes

Microbiol Res. 2018 Oct:215:22-28. doi: 10.1016/j.micres.2018.06.004. Epub 2018 Jun 3.

Authors

Dehong Zheng¹, Zhiyong Zeng², Bingbing Xue², Yaoyao Deng², Ming Sun², Ya-Jie Tang³, Lifang Ruan⁴

Affiliations

¹ State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China; College of Agriculture, Guangxi University, Nanning 530004, PR China.
² State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China.
³ Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan 430068, PR China.
⁴ State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR China. Electronic address: ruanlifang@mail.hzau.edu.cn.

PMID: 30172305
DOI: 10.1016/j.micres.2018.06.004

Abstract

Bacillus thuringiensis has been widely used as a bio-insecticide. However, novel biological activities other than insect toxicity of B. thuringiensis are still underestimated. In this study, a new lipopeptide biosynthesis gene cluster in B. thuringiensis BMB171 was discovered by genome mining and verified by reverse genetics. Thumolycin, the lipopeptide synthesized by this gene cluster, was then isolated and purified. Mass spectrum analysis revealed the molecular mass of thumolycin is 696.51 Da with the predicted molecular formula of C38H64N8O4. Further bioactivities assay showed that thumolycin endowed B. thuringiensis BMB171 with broad spectrum antimicrobial and nematocidal activities.

Keywords: Antimicrobial activity; Bacillus thuringiensis; Lipopeptide; Nematocidal activity.

MeSH terms

Animals
Anti-Infective Agents / chemistry
Anti-Infective Agents / metabolism
Anti-Infective Agents / pharmacology*
Antinematodal Agents / chemistry
Antinematodal Agents / metabolism
Antinematodal Agents / pharmacology
Bacillus thuringiensis / genetics
Bacillus thuringiensis / metabolism*
Bacteria / drug effects
Bacterial Proteins / genetics
Biological Control Agents* / metabolism
Biological Control Agents* / pharmacology
Caenorhabditis elegans / genetics
Gene Deletion
Genes, Bacterial / genetics
Homologous Recombination
Insecticides / chemistry
Insecticides / metabolism
Insecticides / pharmacology
Lipopeptides / biosynthesis*
Lipopeptides / chemistry
Lipopeptides / genetics
Lipopeptides / pharmacology*
Microbial Sensitivity Tests
Molecular Weight
Multigene Family
Nematoda / drug effects*
Pest Control, Biological

Substances

Anti-Infective Agents
Antinematodal Agents
Bacterial Proteins
Biological Control Agents
Insecticides
Lipopeptides