Rio Negro virophage: Sequencing of the near complete genome and transmission electron microscopy of viral factories and particles

Iara Apolinário Borges; Felipe Lopes de Assis; Ludmila Karen Dos Santos Silva; Jônatas Abrahão

doi:10.1016/j.bjm.2018.07.003

Rio Negro virophage: Sequencing of the near complete genome and transmission electron microscopy of viral factories and particles

Braz J Microbiol. 2018 Nov;49 Suppl 1(Suppl 1):260-261. doi: 10.1016/j.bjm.2018.07.003. Epub 2018 Aug 17.

Authors

Iara Apolinário Borges¹, Felipe Lopes de Assis¹, Ludmila Karen Dos Santos Silva¹, Jônatas Abrahão²

Affiliations

¹ Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Microbiologia, Laboratório de Vírus, Belo Horizonte, MG, Brazil.
² Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Microbiologia, Laboratório de Vírus, Belo Horizonte, MG, Brazil. Electronic address: jonatas.abrahao@gmail.com.

Abstract

Rio Negro virophage (RNV) was co-isolated with a strain of mimivirus named sambavirus, from Brazilian Amazon. We report the near complete genome sequence of RNV, the first virophage isolated in Brazil. We also present new microscopical data demonstrating that RNV particles have similar dimensions to that described to sputnik virophages.

MeSH terms

Acanthamoeba / virology*
Brazil
Genome, Viral*
Microscopy, Electron, Transmission
Open Reading Frames
Phylogeny
Togaviridae / genetics*
Togaviridae / isolation & purification
Togaviridae / ultrastructure
Virophages / genetics*
Virophages / isolation & purification
Virophages / ultrastructure