Volatile organic compounds from Hypoxylon anthochroum endophytic strains as postharvest mycofumigation alternative for cherry tomatoes

Abstract

The antifungal activity and chemical composition of the volatile organic compounds (VOCs) produced by four Hypoxylon anthochroum endophytic strains were analyzed. The bioactivity of the VOCs synthesized at different periods of incubation on rice medium was assessed, both in vivo and in vitro, against the phytopathogen Fusarium oxysporum. The in vivo effect was evaluated on cherry tomatoes, while the mechanism of action was determined in vitro analyzing the phytopathogen's growth, respiration and cell membrane permeability. In general, the VOCs from all strains and incubation periods significantly inhibited the growth of F. oxysporum on cherry tomatoes with percentages over 50%. They significantly inhibited the pathogen growth and respiration, and altered the cell membrane permeability and hyphal morphology. The chemical composition was analyzed after solid phase microextraction. In total, 36 VOCs were identified in the four strains, mainly sesquiterpenes and monoterpenes. Among the monoterpenes, eucalyptol had the highest fiber affinity (>60% area) in three of the four strains studied; thus, it could be considered as a chemical marker for H. antochroum. Chemical markers are important for the identification and differentiation of species. The H. anthochroum strains are potential mycofumigation agents against postharvest diseases caused by F. oxysporum.

Keywords: 4-Carene (PubChem CID: 530422); 8-Methylene-tricyclo[3.2.1.0(2,4)]octane (PubChem CID: 142247); Biocontrol; Caryophyllene like (PubChem CID: 5322111); Eucalyptol; Eucalyptol (PubChem CID: 2758); Fungal endophytes; Fusarium oxysporum; Limonene (PubChem CID: 22311); Nodulisporium sp.; Phenylethyl alcohol (PubChem CID: 6054); Sabinene (PubChem CID: 3387-41-5); Thujene (PubChem CID: 17868); Tomato fruit rots; α-Terpineol (PubChem CID: 17100); γ-Terpinene (PubChem CID: 7461).