Antigen host response differences between the animal-type strain and human-clinical Pythium insidiosum isolates used for serological diagnosis in Thailand

Med Mycol. 2019 Jun 1;57(4):519-522. doi: 10.1093/mmy/myy072.

Abstract

The detection of Pythium insidiosum-specific-immunoglobulin-G antibody (Pi-Ab) with enzyme-linked immunosorbent assay (ELISA) test depends on the source of antigen. In this study, the Pi-Ab levels in 140 serum samples from patients with pythiosis were evaluated by ELISA using antigens from 10 P. insidiosum clinical isolates in comparison with antigen from the equine-standard-type strain. The ELISA values (EVs), calculated from antibody levels from serum of patients with pythiosis or other infections versus healthy controls, were significantly higher in the test with clinical-isolates antigen than the standard-equine-type strain (6.0 ± 2.6 vs 4.0 ± 1.7, respectively; P < .0001). ELISA with antigen from human source might be more proper diagnosis test.

Keywords: Pythium insidiosum; Antigenic differences; Serological diagnosis.

Publication types

  • Evaluation Study

MeSH terms

  • Antibodies, Fungal / blood*
  • Antigens, Fungal / immunology*
  • Enzyme-Linked Immunosorbent Assay / methods
  • Humans
  • Immunoglobulin G / blood
  • Pythiosis / diagnosis*
  • Pythium / immunology*
  • Sensitivity and Specificity
  • Serologic Tests / methods*
  • Thailand

Substances

  • Antibodies, Fungal
  • Antigens, Fungal
  • Immunoglobulin G