Compact high-resolution endomicroscopy based on fiber bundles and image stitching

Opt Lett. 2018 Sep 1;43(17):4168-4171. doi: 10.1364/OL.43.004168.

Abstract

In this Letter, we report a compact endomicroscope (ϕ=2.8 mm) based on a fiber bundle and a two-axis piezoelectric tube scanner, achieving a resolution of ∼1 μm and an imaging speed of 30-120 fps. Compared with distal end scanning systems, typical fiber-bundle-based endomicroscopes achieve a more compact envelope (ϕ∼1.5 mm) at the expense of compromised imaging quality. The resolution of fiber-bundle-based systems is largely limited by the diameter of the constituent fibers (ϕ∼5.0 μm), where each fiber serves as a single pixel, i.e., a sampling point, in the imaging system. To retrieve the lost information, we integrate a piezo tube scanner at the tip of the fiber bundle. Next, we rapidly scan the fiber tip over a range of ±2.5 μm and combine the signals obtained at different inter-fiber locations. Direct alignment and feature-based registration methods are applied to register the raw images. Imaging experiments are performed on a resolution target and biological samples to demonstrate the performance enhancement.

MeSH terms

  • Equipment Design
  • Image Processing, Computer-Assisted
  • Microscopy / instrumentation*
  • Optical Fibers*
  • Pinus
  • Plant Leaves
  • Pollen
  • Signal-To-Noise Ratio*