To investigate the effect of concentrated growth factor (CGF) on proliferation and differentiation in Beagle adipose-derived stem cells (ADSCs). Methods: ADSCs were isolated from adipose tissue of healthy Beagles and cultured. The multi-directional differentiation potential of ADSCs was identified. The ADSCs were assigned to a CGF group and a control group. The rate of proliferation was analyzed by CCK-8 assay. The osteogenic differentiation capability was detected by ALP staining after the osteoinduction. Bone formation-related gene expression was detected by RT-PCR. Results: CGF promoted the proliferation of ADSCs in vitro. ADSCs in the CGF group showed higher level of ALP activity than that in the control group (P<0.05). CGF stimulated the expression of the genes associated with osteogenesis, such as Col-I and Runx2. Conclusion: CGF can promote the proliferation and osteogenic differentiation in ADSCs in vitro.
目的：分析自体浓缩生长因子(concentrated growth factor，CGF)对比格犬脂肪干细胞(adipose-derived stem cells，ADSCs)体外增殖及成骨向分化的影响。方法：取健康比格犬脂肪组织进行分离、培养ADSCs并鉴定其多向分化潜能，细胞计数试剂盒8(cell counting kit-8，CCK-8)检测细胞增殖。将ADSCs分为CGF组及对照组，对两组细胞进行成骨诱导后分别检测其碱性磷酸酶(ALP)活性，RT-PCR法检测I型胶原(Col-I)，Runx2等成骨相关基因表达。结果：CGF组细胞增殖能力明显高于对照组(P<0.05)；经成骨诱导的ADSCs在CGF作用下ALP活性明显增强(P<0.05)，Col-I，Runx2等成骨相关基因的表达亦明显高于对照组(P<0.05)。 结论：CGF能够促进ADSCs体外增殖和成骨向分化。.