Cleavable and thermo-responsive hybrid nanoparticles for on-demand drug delivery

Isabel Ortiz de Solorzano; Teresa Alejo; Miriam Abad; Carlos Bueno-Alejo; Gracia Mendoza; Vanesa Andreu; Silvia Irusta; Victor Sebastian; Manuel Arruebo

doi:10.1016/j.jcis.2018.08.069

Cleavable and thermo-responsive hybrid nanoparticles for on-demand drug delivery

J Colloid Interface Sci. 2019 Jan 1:533:171-181. doi: 10.1016/j.jcis.2018.08.069. Epub 2018 Aug 23.

Authors

Isabel Ortiz de Solorzano¹, Teresa Alejo², Miriam Abad³, Carlos Bueno-Alejo⁴, Gracia Mendoza², Vanesa Andreu², Silvia Irusta⁵, Victor Sebastian⁵, Manuel Arruebo⁵

Affiliations

¹ Department of Chemical Engineering, Aragon Institute of Nanoscience (INA), University of Zaragoza, Campus Río Ebro-Edificio I+D, C/ Poeta Mariano Esquillor S/N, 50018 Zaragoza, Spain; Aragon Health Research Institute (IIS Aragón), 50009 Zaragoza, Spain; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine, CIBER-BBN, 28029 Madrid, Spain. Electronic address: isaortiz@unizar.es.
² Department of Chemical Engineering, Aragon Institute of Nanoscience (INA), University of Zaragoza, Campus Río Ebro-Edificio I+D, C/ Poeta Mariano Esquillor S/N, 50018 Zaragoza, Spain; Aragon Health Research Institute (IIS Aragón), 50009 Zaragoza, Spain.
³ Departamento de Química Orgánica, Facultad de Ciencias, Instituto de Ciencia de Materiales de Aragón (ICMA), Universidad de Zaragoza-CSIC, 50009 Zaragoza, Spain.
⁴ Department of Chemical Engineering, Aragon Institute of Nanoscience (INA), University of Zaragoza, Campus Río Ebro-Edificio I+D, C/ Poeta Mariano Esquillor S/N, 50018 Zaragoza, Spain; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine, CIBER-BBN, 28029 Madrid, Spain.
⁵ Department of Chemical Engineering, Aragon Institute of Nanoscience (INA), University of Zaragoza, Campus Río Ebro-Edificio I+D, C/ Poeta Mariano Esquillor S/N, 50018 Zaragoza, Spain; Aragon Health Research Institute (IIS Aragón), 50009 Zaragoza, Spain; Networking Research Center on Bioengineering, Biomaterials and Nanomedicine, CIBER-BBN, 28029 Madrid, Spain.

PMID: 30153594
DOI: 10.1016/j.jcis.2018.08.069

Abstract

By combining the photothermal ability of copper sulphide nanoparticles (NPs) upon excitation with Near Infrared (NIR) Light and the thermo-responsive properties of the homemade oligo (ethylene glycol) methyl ether methacrylate copolymer we have obtained fragmentable nanocomposites able to release a carried drug on-demand after NIR-light triggering. A complete physico-chemical characterization of the resulting nanoparticles has been carried out and their degradation assessed at different temperatures. Herein, we have also evaluated the drug loading capacity of those nanoparticles and the temperature dependence in their drug release kinetics using bupivacaine hydrochloride as a model drug. For those hybrid nanoparticles, subcytotoxic doses on four different cell lines and their potential interference in cell metabolism, induction of apoptosis, and cell cycle have been evaluated by Alamar Blue fluorometry and flow cytometry.

Keywords: Bupivacaine; Cleavable nanoparticles; Drug delivery; NIR; Photothermal; Thermosensitive polymer.

MeSH terms

Anesthetics, Local / chemistry
Anesthetics, Local / pharmacology*
Animals
Apoptosis / drug effects
Bupivacaine / chemistry
Bupivacaine / pharmacology*
Cell Cycle / drug effects
Cell Survival / drug effects
Cells, Cultured
Copper / chemistry*
Drug Delivery Systems*
Humans
Infrared Rays
Mice
Nanoparticles / chemistry*
Particle Size
Sulfides / chemistry*
Surface Properties
Temperature*

Substances

Anesthetics, Local
Sulfides
cuprous sulfide
Copper
Bupivacaine