A new electrochemical aptasensor for sensitive assay of a protein based on the dual-signaling electrochemical ratiometric method and DNA walker strategy

Caixia Zhu; Mengyue Liu; Xiaoyu Li; Xiaohua Zhang; Jinhua Chen

doi:10.1039/c8cc05829f

A new electrochemical aptasensor for sensitive assay of a protein based on the dual-signaling electrochemical ratiometric method and DNA walker strategy

Chem Commun (Camb). 2018 Sep 11;54(73):10359-10362. doi: 10.1039/c8cc05829f.

Authors

Caixia Zhu¹, Mengyue Liu, Xiaoyu Li, Xiaohua Zhang, Jinhua Chen

Affiliation

¹ State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, 410082, P. R. China. chenjinhua@hnu.edu.cn.

PMID: 30152501
DOI: 10.1039/c8cc05829f

Abstract

Herein, a new electrochemical aptamer-based biosensor for highly sensitive assay of thrombin has been developed based on the dual-signaling electrochemical ratiometric method and the DNA walker strategy, and shows a low detection limit of about 56 fM.

MeSH terms

Aptamers, Nucleotide / chemistry*
Biosensing Techniques
DNA / chemistry*
Dielectric Spectroscopy / instrumentation*
Dielectric Spectroscopy / methods
Limit of Detection
Thrombin / analysis*

Substances

Aptamers, Nucleotide
DNA
Thrombin