Enzyme-Embedded Metal-Organic Framework Colloidosomes via an Emulsion-Based Approach

Guixian Zhu; Mizhen Zhang; Yang Bu; Lidan Lu; Xiaoping Lou; Lianqing Zhu

doi:10.1002/asia.201800976

Enzyme-Embedded Metal-Organic Framework Colloidosomes via an Emulsion-Based Approach

Chem Asian J. 2018 Oct 4;13(19):2891-2896. doi: 10.1002/asia.201800976. Epub 2018 Aug 27.

Authors

Guixian Zhu¹, Mizhen Zhang¹, Yang Bu², Lidan Lu³, Xiaoping Lou¹, Lianqing Zhu^{1

4}

Affiliations

¹ Beijing Key Laboratory for Optoelectronics Measurement Technology, Beijing Information Science and Technology University, No. 12, Xiaoying East Road, Beijing, 100016, China.
² College of Materials Sciences and Optoelectronics, University of Chinese Academy of Sciences, No. 19(A) Yuquan Road, Beijing, 100049, China.
³ School of Precision Instrument and Opto-electronics Engineering, Tianjin University, No.92 Weijin Road, Tianjin, 300072, China.
⁴ School of Instrument and Opto-electronics Engineering, Hefei University of Technology, No.193 Xitun Road, Anhui, 230009, China.

PMID: 30151959
DOI: 10.1002/asia.201800976

Abstract

Improving the activity and stability of enzymes is significant in enzyme immobilization. Here a facile approach to prepare ring-like ZIF-8 colloidosomes and spherical catalase-embedded ZIF-8 colloidosomes is developed via one-step emulsion-based technique at the water/butanol interface. The influence of the concentrations of ZIF-8 nanocrystals and Pluronic F127 as well as the oil-water ratio was investigated. Compared with in situ biomineralization, the colloidosomes prepared via the pickering emulsion method show successful encapsulation of positively charged enzymes. By using catalase as an immobilized model, the immobilized catalase exhibits high biocatalytic activity, stability and recyclability compared with free catalase.

Keywords: ZIF-8 nanocrystals; biocatalysis; colloidosomes; enzyme immobilization; pickering emulsion.

Abstract

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