Evaluating Blood Cell Populations in Xenopus Using Flow Cytometry and Differential Counts by Cytospin

Jacques Robert; Eva-Stina Edholm; Francisco De Jesus Andino

doi:10.1007/978-1-4939-8784-9_19

Evaluating Blood Cell Populations in Xenopus Using Flow Cytometry and Differential Counts by Cytospin

Methods Mol Biol. 2018:1865:265-273. doi: 10.1007/978-1-4939-8784-9_19.

Authors

Jacques Robert¹, Eva-Stina Edholm^{2

3}, Francisco De Jesus Andino²

Affiliations

¹ Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, NY, USA. Jacques_Robert@URMC.Rochester.edu.
² Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, NY, USA.
³ The Norwegian College of Fishery Science, University of Tromsø, Tromsø, Norway.

PMID: 30151773
DOI: 10.1007/978-1-4939-8784-9_19

Abstract

Analyses by flow cytometry and differential counts by cytospin of peripheral blood leukocytes are two reliable and convenient methods used to assess the immune status and immune responses in the amphibian Xenopus. Here, we describe these methods and discuss their challenges and limitations.

Keywords: Amphibian; Aquatic vertebrates; Comparative immunology; Developmental immunology.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Animals
Blood Cell Count / methods*
Blood Cells / cytology*
Cell Separation
Centrifugation
Flow Cytometry / methods*
Xenopus laevis / metabolism*