Emergence of IncX4 plasmids encoding mcr-1 in a clinical isolate of Klebsiella pneumoniae in Japan

Tatsuya Tada; Kohei Uechi; Isamu Nakasone; Masashi Nakamatsu; Kazuhito Satou; Takashi Hirano; Teruo Kirikae; Jiro Fujita

doi:10.1016/j.ijid.2018.08.011

Emergence of IncX4 plasmids encoding mcr-1 in a clinical isolate of Klebsiella pneumoniae in Japan

Int J Infect Dis. 2018 Oct:75:98-100. doi: 10.1016/j.ijid.2018.08.011. Epub 2018 Aug 24.

Authors

Tatsuya Tada¹, Kohei Uechi², Isamu Nakasone³, Masashi Nakamatsu⁴, Kazuhito Satou⁵, Takashi Hirano⁶, Teruo Kirikae⁷, Jiro Fujita⁸

Affiliations

¹ Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan.
² Division of Clinical Laboratory and Blood Transfusion, University Hospital of the Ryukyus, Okinawa, Japan; Department of Infectious Diseases, Respiratory, and Digestive Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan.
³ Control and Prevention of Infectious Disease, University Hospital of the Ryukyus, Okinawa, Japan.
⁴ Department of Infectious Diseases, Faculty of the Ryukyus, Okinawa, Japan.
⁵ Okinawa Institute of Advanced Science, Okinawa, Japan; Department of Genome Medicine, Research Institute, National Center for Children Health and Development, Tokyo, Japan.
⁶ Okinawa Institute of Advanced Science, Okinawa, Japan.
⁷ Department of Microbiology, Juntendo University School of Medicine, Tokyo, Japan. Electronic address: t-kirikae@juntendo.ac.jp.
⁸ Department of Infectious Diseases, Respiratory, and Digestive Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa, Japan.

PMID: 30149089
DOI: 10.1016/j.ijid.2018.08.011

Abstract

The mcr-1 gene encodes a phosphoethanolamine transferase that confers resistance to colistin by transferring phosphoethanolamine to lipid A. A 33-kb IncX4 plasmid harboring mcr-1 was detected in a Klebsiella pneumoniae isolate and two Escherichia coli isolates, and a 66-kb IncI2 plasmid was detected in three E. coli isolates in hospitals in Okinawa, Japan.

Keywords: Colistin resistance; IncX4 plasmid harboring mcr-1; MCR-1 producing Klebsiella pneumoniae.

MeSH terms

Anti-Bacterial Agents / pharmacology
Colistin / pharmacology
Drug Resistance, Bacterial
Escherichia coli / enzymology
Escherichia coli / genetics
Escherichia coli / isolation & purification
Escherichia coli Proteins / genetics
Escherichia coli Proteins / metabolism
Humans
Japan
Klebsiella Infections / microbiology
Klebsiella pneumoniae / drug effects
Klebsiella pneumoniae / enzymology
Klebsiella pneumoniae / genetics
Klebsiella pneumoniae / isolation & purification*
Methyltransferases / genetics
Methyltransferases / metabolism*
Plasmids / genetics*
Plasmids / metabolism

Substances

Anti-Bacterial Agents
Escherichia coli Proteins
MCR-1 protein, E coli
Methyltransferases
phosphoethanolamine methyltransferase
Colistin