Exopolysaccharide production by an indigenous isolate Pseudomonas stutzeri XP1 and its application potential in enhanced oil recovery

Feng Zhao; Chao Guo; Qingfeng Cui; Qinqin Hao; Jianlong Xiu; Siqin Han; Ying Zhang

doi:10.1016/j.carbpol.2018.07.038

Exopolysaccharide production by an indigenous isolate Pseudomonas stutzeri XP1 and its application potential in enhanced oil recovery

Carbohydr Polym. 2018 Nov 1:199:375-381. doi: 10.1016/j.carbpol.2018.07.038. Epub 2018 Jul 18.

Authors

Feng Zhao¹, Chao Guo², Qingfeng Cui³, Qinqin Hao², Jianlong Xiu³, Siqin Han¹, Ying Zhang⁴

Affiliations

¹ CAS Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences (CAS), Shenyang, Liaoning Province, 110016, China.
² CAS Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences (CAS), Shenyang, Liaoning Province, 110016, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
³ Institute of Porous Flow & Fluid Mechanics, Research Institute of Petroleum Exploration & Development, Langfang, Hebei Province, 065007, China.
⁴ CAS Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences (CAS), Shenyang, Liaoning Province, 110016, China. Electronic address: yzhang@iae.ac.cn.

PMID: 30143141
DOI: 10.1016/j.carbpol.2018.07.038

Abstract

In this study, a rapid and efficient method for screening biopolymer producers was established using 96-well plates. An indigenous biopolymer producer Pseudomonas stutzeri XP1 was isolated from Xinjiang oil reservoirs, China. Strain XP1 can grow and produce 16 g/l biopolymer using corn starch and nitrate. Produced biopolymer increased culture viscosity up to 2384 mPa s. Biopolymer showed rheological properties and pseudo-plastic behavior. The viscosity of 8 g/l biopolymer solution kept higher than 25 mPa s at 20-50 °C and pH values (5-9) and increased to 7600 mPa s with NaCl concentrations increasing to 2%. Gel permeation chromatography data showed that the biopolymer average molecular mass was 1.65 × 10⁶ Da. Gas chromatography revealed that the monosaccharide composition in biopolymer was glucose. Core flooding experiments revealed that extra 13.56% of oil was recovered by in situ biopolymer production of strain XP1. Properties of strain XP1 and the biopolymer produced make them promising for enhanced oil recovery.

Keywords: Biopolymer; Exopolysaccharide; Microbial enhanced oil recovery; Pseudomonas stutzeri; Rheological property.