Structure characterization of the mannofucogalactan isolated from fruit bodies of Quinine conk Fomitopsis officinalis

Victoria V Golovchenko; Daria S Khramova; Naranmandakh Shinen; Ganbaatar Jamsranjav; Alexander O Chizhov; Alexander S Shashkov

doi:10.1016/j.carbpol.2018.06.103

Structure characterization of the mannofucogalactan isolated from fruit bodies of Quinine conk Fomitopsis officinalis

Carbohydr Polym. 2018 Nov 1:199:161-169. doi: 10.1016/j.carbpol.2018.06.103. Epub 2018 Jun 26.

Authors

Victoria V Golovchenko¹, Daria S Khramova², Naranmandakh Shinen³, Ganbaatar Jamsranjav⁴, Alexander O Chizhov⁵, Alexander S Shashkov⁵

Affiliations

¹ Institute of Physiology, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, 50, Pervomaiskaya Str., Syktyvkar, 167982, Russia. Electronic address: lemnan@mail.ru.
² Institute of Physiology, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, 50, Pervomaiskaya Str., Syktyvkar, 167982, Russia.
³ National University of Mongolia, School of Life Sciences, Ulaanbaatar-51, Mongolia.
⁴ Institute of Chemistry and Chemical Technology, The Mongolian Academy of Sciences, Ulaanbaatar-51, Mongolia.
⁵ N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 47, Leninsky Prospect, Moscow, 119991, Russia.

PMID: 30143116
DOI: 10.1016/j.carbpol.2018.06.103

Abstract

The mannofucogalactan as a major component of water extract was obtained from fruit bodies of Fomitopsis officinalis by extraction with boiling water followed by deproteination, decoloration, and purification using anion-exchange chromatography and size exclusion chromatography. Its structure was characterized using the data of monosaccharide composition, methylation analysis, one- and two-dimensional NMR spectroscopy. The studied polysaccharide was a branched mannofucogalactan with a backbone composed of partially 3-O-methylated 1,6-O-linked α-D-galactopyranosyl residues. Almost every second residue in the backbone was substituted at O-2 by 3-O-α-D-mannopyranosyl-α-L-fucopyranosyl and β-D-galactopyranosyl residues. The non-reducing terminal α-L-fucopyranosyl units, which were identified by GC-MS analyses, appeared to be the part of mannofucogalactan side chains also.

Keywords: Fomitopsis officinalis; Mannofucogalactan; Medicinal fungi; Spectroscopy NMR; Structure.