EM Tomography of Meiotic LINC Complexes

Marie-Christin Spindler; Frederik Helmprobst; Christian Stigloher; Ricardo Benavente

doi:10.1007/978-1-4939-8691-0_1

EM Tomography of Meiotic LINC Complexes

Methods Mol Biol. 2018:1840:3-15. doi: 10.1007/978-1-4939-8691-0_1.

Authors

Marie-Christin Spindler¹, Frederik Helmprobst², Christian Stigloher², Ricardo Benavente³

Affiliations

¹ Department of Cell and Developmental Biology, Biocenter, University of Würzburg, Würzburg, Germany.
² Imaging Core Facility, Biocenter, University of Würzburg, Würzburg, Germany.
³ Department of Cell and Developmental Biology, Biocenter, University of Würzburg, Würzburg, Germany. benavente@biozentrum.uni-wuerzburg.de.

PMID: 30141033
DOI: 10.1007/978-1-4939-8691-0_1

Abstract

Electron microscope (EM) tomography is a powerful technique that enables the three-dimensional analysis of subcellular structures at high resolution. We have applied this method to the quantitative analysis of LINC complex distribution and interaction with the cytoskeleton in meiotic cells from male mice. In this chapter, we describe methods to generate and analyze the tomograms.

Keywords: Cytoskeleton; EM tomography; LINC complex; Meiosis; Microtubules; Nuclear envelope; Telomere.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cytoskeleton / ultrastructure*
Electron Microscope Tomography* / methods
Male
Meiosis*
Mice
Nuclear Envelope / ultrastructure*
Telomere / ultrastructure*