Kv12.1 channels are not sensitive to GqPCR-triggered activation of phospholipase Cβ

Marlen Dierich; Michael G Leitner

doi:10.1080/19336950.2018.1475783

K_v12.1 channels are not sensitive to G_qPCR-triggered activation of phospholipase Cβ

Channels (Austin). 2018;12(1):228-239. doi: 10.1080/19336950.2018.1475783.

Authors

Marlen Dierich¹, Michael G Leitner^{1

2}

Affiliations

¹ a Department of Neurophysiology , Institute of Physiology and Pathophysiology, Philipps-University Marburg , Marburg , Germany.
² b Division of Physiology, Department of Physiology and Medical Physics , Medical University of Innsbruck , Innsbruck , Austria.

Abstract

K_v12.1 K⁺ channels are expressed in several brain areas, but no physiological function could be attributed to these subunits so far. As genetically-modified animal models are not available, identification of native K_v12.1 currents must rely on characterization of distinct channel properties. Recently, it was shown in Xenopus laevis oocytes that K_v12.1 channels were modulated by membrane PI(4,5)P₂. However, it is not known whether these channels are also sensitive to physiologically-relevant PI(4,5)P₂ dynamics. We thus studied whether K_v12.1 channels were modulated by activation of phospholipase C β (PLCβ) and found that they were insensitive to receptor-triggered depletion of PI(4,5)P₂. Thus, K_v12.1 channels add to the growing list of K⁺ channels that are insensitive to PLCβ signaling, although modulated by PI(4,5)P₂ in Xenopus laevis oocytes.

Keywords: Ci-VSP; GqPCR; KCNQ; Kv12; Kv7; M current; PI(3,4,5)P3; PI(4,5)P2; mode shift; phospholipase C.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
CHO Cells
Cells, Cultured
Cricetulus
Humans
Phosphatidylinositol 4,5-Diphosphate / metabolism
Phospholipase C beta / metabolism*
Potassium Channels / metabolism
Receptors, G-Protein-Coupled / metabolism*

Substances

Phosphatidylinositol 4,5-Diphosphate
Potassium Channels
Receptors, G-Protein-Coupled
Phospholipase C beta

Grants and funding

This work was funded by Research Grants of the University Medical Center Giessen and Marburg (UKGM 17/2013; UKGM 13/2016 to M.G.L.) and by the German Research Foundation (DFG Priority Program 1608:”Ultrafast and temporally precise information processing: Normal and dysfunctional hearing”, [LE 3600/1-1 to MGL]).