Necroptosis in macrophages is increasingly being recognized as a significant contributor to the inflammation seen in many pathologies. Here we describe a well-known method to obtain quiescent, mature macrophages that can be used to study the molecular mechanisms that regulate necroptosis. We also describe two ways to quantify this form of programmed cell death.
Keywords: ATP viability assay; Bone marrow-derived macrophages; Cell death; Luminescence; Necroptosis; Propidium iodide exclusion.