Rational design of artificial microstructured metamaterials with advanced mechanical and physical properties that are not accessible in nature materials is very important. Making use of node rotation and ligament bending deformation features of chiral materials, two types of innovative 3D chiral metamaterials are proposed, namely chiral- chiral- antichiral and chiral- antichiral- antichiral metamaterials. In-situ compression and uniaxial tensile tests are performed for studying the mechanical properties and deformation mechanisms of these two types of 3D chiral metamaterials. Novel deformation mechanisms along different directions are explored and analyzed, such as: uniform spatial rotation deformation, tensile-shearing directed (compression-shearing directed), tensile-expansion directed (compression-shrinkage directed) deformation mechanisms of 3D chiral metamaterials, and competitions between different types of deformation mechanisms are discussed. The proposed 3D chiral metamaterials represents a series of metamaterials with robust microstructures design feasibilities.