Livestock slurry stored in ponds is an important source of methane emission, which is influenced by environmental factors. In this study, the effect of slurry properties and temperature on methane flux and methanogen community composition was investigated. The methanogen community composition in swine slurry was more sensitive to temperature and significantly different from that of cattle slurry (ANOSIM, P < 0.05), especially for the phylotypes affiliated with Methanobrevibacter, Methanocorpusculaceae and Methanocorpusculum. These different methanogen communities partially accounted for the differences in methane flux between swine and cattle slurries. Methanogen abundance seemed to not be affected by slurry properties or temperature, but the mcrA (encoding the alpha subunit of methyl coenzyme M reductase) transcript/gene ratio was significantly increased at 30°C and was higher in swine slurry than in cattle slurry (t-test, P < 0.05). This study reveals that higher temperatures increased methane production by promoting the transcription of mcrA rather than by increasing methanogen cell numbers.
Keywords: Methanogen; mcrA; methane flux; stored livestock slurry; temperature.