Microinjection of DNA Constructs into Xenopus Embryos for Gene Misexpression and cis-Regulatory Module Analysis

Yuuri Yasuoka; Masanori Taira

doi:10.1101/pdb.prot097279

Microinjection of DNA Constructs into Xenopus Embryos for Gene Misexpression and cis-Regulatory Module Analysis

Cold Spring Harb Protoc. 2019 Jan 2;2019(1). doi: 10.1101/pdb.prot097279.

Authors

Yuuri Yasuoka¹, Masanori Taira²

Affiliations

¹ Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate University, Onna-son, Okinawa 904-0495, Japan.
² Department of Biological Sciences, Graduate School of Science, University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan m_taira@bs.s.u-tokyo.ac.jp m-taira.183@g.chuo-u.ac.jp.

PMID: 30131366
DOI: 10.1101/pdb.prot097279

Abstract

Introducing exogenous DNA into an embryo can promote misexpression of a gene of interest via transcription regulated by an attached enhancer-promoter. This protocol describes plasmid DNA microinjection into Xenopus embryos for misexpression of genes after zygotic gene expression begins. It also describes a method for coinjecting a reporter plasmid with mRNA or antisense morpholinos to perform luciferase reporter assays, which are useful for quantitative analysis of cis-regulatory sequences responding to endogenous or exogenous stimuli in embryos.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
DNA / administration & dosage*
Gene Expression Regulation, Developmental*
Microinjections / methods*
Plasmids / administration & dosage
Xenopus / embryology*

Substances

DNA