Chemical diversity from the Tibetan Plateau fungi Penicillium kongii and P. brasilianum

Zhi-Guo Liu; Li Bao; Hong-Wei Liu; Jin-Wei Ren; Wen-Zhao Wang; Long Wang; Wei Li; Wen-Bing Yin

doi:10.1080/21501203.2017.1331937

Chemical diversity from the Tibetan Plateau fungi Penicillium kongii and P. brasilianum

Mycology. 2017 Jun 12;9(1):10-19. doi: 10.1080/21501203.2017.1331937. eCollection 2018.

Authors

Zhi-Guo Liu^{1

2}, Li Bao¹, Hong-Wei Liu¹, Jin-Wei Ren¹, Wen-Zhao Wang¹, Long Wang¹, Wei Li^{1

2}, Wen-Bing Yin^{1

2

3}

Affiliations

¹ State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
² State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
³ Savaid Medical School, University of Chinese Academy of Sciences, Beijing, China.

Abstract

Two new secondary metabolites, kongiilines A and B (1, 7), and two asperphenamate derivatives, asperphenamates B and C (5-6), together with 16 known compounds (2-4, 8-20), were isolated from Tibetan Plateau fungi Penicillium kongii and Penicillium brasilianum. This is the first report on asperphenamates B and C as naturally occurring compounds, and that aspterric acid is isolated from P. brasilianum for the first time. Their structures were elucidated by different spectroscopic techniques including high-resolution electrospray ionisation mass spectrum, 1D nuclear magnetic resonance (NMR), and 2D NMR as well as electronic circular dichroism. Compounds 4, 5, and 10 exhibited cytotoxicity activities against human colon carcinoma HCT116 cell line with IC₅₀ values of 88.16, 77.68, and 36.92 μM, respectively. Fungi from Tibetan Plateau represent important and rich resources for the investigation of new chemicals.

Keywords: Antitumour cell; Tibet; moulds; new compounds; structural elucidation.

Grants and funding

This work was supported by the National Natural Science Foundation of China under: [Grant Number 81502964] to Z.G.L. and [Grant Number 31270539] to L.W., the State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College under Grant GTZK201506. W.B.Y. is a scholar of the “100 Talents Project” of CAS.