Flavonoid glycoside degradation can proceed through two alternative enzymatic pathways: one that is mediated by monoglycosidases, and the other catalyzed by a diglycosidase. β-Diglycosidase performs the flavonoid deglycosylation in a single reaction. The characterized β-diglycosidase activities recognize the following disaccharidic sugar moieties: β-primeverose, acuminose, vicianose, and β-rutinose. The present paper reviews the biochemical characteristics and potential industrial applications of microbial β-diglycosidases that break down plant diglycoconjugated flavonoids.
Keywords: Archaea; Bacteria; Enzyme catalysis; Fungi; β-Diglycosidase.