New Ansamycins from the Deep-Sea-Derived Bacterium Ochrobactrum sp. OUCMDZ-2164

Yaqin Fan; Cong Wang; Liping Wang; Arthit Chairoungdua; Pawinee Piyachaturawat; Peng Fu; Weiming Zhu

doi:10.3390/md16080282

New Ansamycins from the Deep-Sea-Derived Bacterium Ochrobactrum sp. OUCMDZ-2164

Mar Drugs. 2018 Aug 15;16(8):282. doi: 10.3390/md16080282.

Authors

Yaqin Fan^{1

2}, Cong Wang^{3

4}, Liping Wang⁵, Arthit Chairoungdua⁶, Pawinee Piyachaturawat⁷, Peng Fu^{8

9}, Weiming Zhu^{10

11

12}

Affiliations

¹ Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China. fanyaqin.826@163.com.
² Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266003, China. fanyaqin.826@163.com.
³ Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China. wangcong123206@163.com.
⁴ Guangxi Key Laboratory of Chemistry and Engineering of Forest Products, School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, China. wangcong123206@163.com.
⁵ State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China. lipingw2006@163.com.
⁶ Department of Physiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand. arthit.chi@mahidol.ac.th.
⁷ Department of Physiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand. pawinee.pia@mahidol.ac.th.
⁸ Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China. fupeng@ouc.edu.cn.
⁹ Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266003, China. fupeng@ouc.edu.cn.
¹⁰ Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China. weimingzhu@ouc.edu.cn.
¹¹ Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266003, China. weimingzhu@ouc.edu.cn.
¹² State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China. weimingzhu@ouc.edu.cn.

Abstract

Two new ansamycins, trienomycins H (1) and I (2), together with the known trienomycinol (3), were isolated from the fermentation broth of the deep-sea-derived bacterium Ochrobactrum sp. OUCMDZ-2164. Their structures, including their absolute configurations, were elucidated based on spectroscopic analyses, ECD spectra, and Marfey's method. Compound 1 exhibited cytotoxic effects on A549 and K562 cell lines with IC₅₀ values of 15 and 23 μM, respectively.

Keywords: Ochrobactrum sp.; ansamycins; cytotoxic activity; deep-sea-derived bacterium; trienomycins.

MeSH terms

A549 Cells
Aquatic Organisms / chemistry*
Cell Line, Tumor
Fermentation / physiology
Humans
K562 Cells
Lactams, Macrocyclic / chemistry*
Ochrobactrum / chemistry*
Spectrum Analysis

Substances

Lactams, Macrocyclic