[Transcriptome analysis reveals candidate genes involved in flavonols biosyhthesis in Sophora japonica]

Yuan Pan; Da-Xia Chen; Xu-Hong Song; Long-Yun Li

doi:10.19540/j.cnki.cjcmm.20180510.004

[Transcriptome analysis reveals candidate genes involved in flavonols biosyhthesis in Sophora japonica]

Zhongguo Zhong Yao Za Zhi. 2018 Jul;43(13):2682-2689. doi: 10.19540/j.cnki.cjcmm.20180510.004.

[Article in Chinese]

Authors

Yuan Pan^{1

2

3}, Da-Xia Chen^{1

2

3}, Xu-Hong Song^{1

2

3}, Long-Yun Li^{1

2

3}

Affiliations

¹ Chongqing Academy of Chinese Material Medica， Chongqing 400065, China.
² Chongqing Subcenters of Traditional Chinese Medicine Resource Center and China Academy of Chinese Medical Sciences， Chongqing 400065, China.
³ Chongqing Research Center of Techniques and Engineering for Chinese Material Medica Fine Varierty Breeding and Evaluation, Chongqing 400065, China.

PMID: 30111017
DOI: 10.19540/j.cnki.cjcmm.20180510.004

Abstract

In order to study the pathways of biosynthesis of flavonoids in Sophora japonica, 113 797 unigenes were obtained by Trinity software, with an average length of 803 bp, of which 72 752 unigenes were obtained from the database by high-throughput sequencing, and a total of 38 891 SSR loci were searched. Through the metabolic pathway analysis, we found that there were 135 unigenes involved in the biosynthesis of flavonoids and 959 unigene involved in other secondary metabolic pathways. Further analysis of genes involved in rutin biosynthesis revealed that 24 were associated with CHS, 52 were associated with FLS, and 11 were associated with UFGT. The obtained data of S. japonica transcriptome lays the foundation for studying the pathways of biosynthesis of flavonoids in S. japonica and provides theoretical basis for the formation of the quality of S. japonica.

Keywords: Sophora japonica; flavonoids; high throughput sequencing; transcriptome.

MeSH terms

Flavonols
Gene Expression Profiling
High-Throughput Nucleotide Sequencing
Sophora*
Transcriptome

Substances

Flavonols