On the systematics of some marine haploporids (Trematoda) with the description of a new species of Megasolena Linton, 1910

Michael J Andres; Eric E Pulis; Stephen S Curran; Robin M Overstreet

doi:10.1016/j.parint.2018.08.002

On the systematics of some marine haploporids (Trematoda) with the description of a new species of Megasolena Linton, 1910

Parasitol Int. 2018 Dec;67(6):805-815. doi: 10.1016/j.parint.2018.08.002. Epub 2018 Aug 12.

Authors

Michael J Andres¹, Eric E Pulis², Stephen S Curran³, Robin M Overstreet³

Affiliations

¹ Division of Coastal Sciences, The University of Southern Mississippi, 703 East Beach Drive, Ocean Springs, MS 39564, USA. Electronic address: michael.andres@usm.edu.
² Division of Coastal Sciences, The University of Southern Mississippi, 703 East Beach Drive, Ocean Springs, MS 39564, USA; Department of Science and Mathematics, Northern State University, 1200 South Jay Street, Aberdeen, SD 57401, USA.
³ Division of Coastal Sciences, The University of Southern Mississippi, 703 East Beach Drive, Ocean Springs, MS 39564, USA.

PMID: 30110653
DOI: 10.1016/j.parint.2018.08.002

Abstract

Megasolena mikra sp. nov. is described from the queen angelfish, Holacanthus ciliaris (Linnaeus), off Florida, USA. The new species can be differentiated from all other species of Megasolena Linton, 1910 except Megasolena littoralis Muñoz, George-Nascimento, and Bray, 2017 in possessing testes that are smaller in diameter than the ovary. The new species can be differentiated from M. littoralis in lacking tegumental spines and possessing oral sucker papillae. Molecular data are provided for two species each of Cadenatella Dollfus, 1946, Hapladena Linton, 1910, and Megasolena Linton, 1910. Bayesian inference analysis of concatenated internal transcribed spacer region-2 (ITS2) and partial 28S rDNA sequences of 50 haploporoids revealed 1) a monophyletic Atractotrematidae Yamaguti, 1939 sister to the rest of the haploporoids tested; 2) a paraphyletic Megasoleninae Manter, 1935 - if Hapladena is included; and 3) a monophyletic Cadenatellinae Gibson and Bray, 1982 sister to the 'mugilid' haploporids. The 'mugilid' haploporids formed a monophyletic clade consisting of the subfamilies Chalcinotrematinae Overstreet and Curran, 2005, Forticulcitinae Blasco-Costa, Balbuena, Kostadinova, and Olson, 2009, Haploporinae Nicoll, 1914, and Waretrematinae Srivastava, 1937. Based on our analysis we restrict the Megasoleninae to include Megasolena, Vitellibaculum Montgomery, 1957, and Metamegasolena Yamaguti, 1970, all of which have species with two testes. To accommodate the former megasolenine taxa with a single testis, we erect the Hapladeninae subf. nov. for species in Hapladena and tentatively, Myodera Montgomery, 1957. Our results further support that haploporoids had a common marine ancestor with two testes, and that members of the Haploporoidea Nicoll, 1914 underwent diversification following a shift from a primarily marine life history with eupercarian hosts to a more euryhaline one with diadromous hosts (namely mullet).

Keywords: 28S rRNA; Cadenatella; Hapladena; Haploporidae; ITS region; Phylogeny.

MeSH terms

Animal Distribution
Animals
DNA, Helminth / genetics*
DNA, Ribosomal Spacer / genetics
Florida
Host-Parasite Interactions*
Perciformes / parasitology*
Phylogeny
RNA, Ribosomal, 28S / genetics
Trematoda / anatomy & histology
Trematoda / classification*
Trematoda / genetics

Substances

DNA, Helminth
DNA, Ribosomal Spacer
RNA, Ribosomal, 28S