Synthetic biology is an emergent field of research whose aim is to make biology an engineering discipline, thus permitting to design, control, and standardize biological processes. Synthetic biology is therefore expected to boost the development of biotechnological processes such as protein production and enzyme engineering, which can be significantly relevant for lipases and esterases.
Keywords: CRISPR-Cas9; Computer-aided design (CAD); Esterases; Flux balance analysis (FBA); Genome-scale modelling (GEM); Lipases; Metabolic engineering; Synthetic biology; TALEN; ZFN.