Abstract
We explored quenching of the PlcR-PapR quorum-sensing system in Bacillus cereus. We generated PapR7-peptidic derivatives that inhibit this system and thus the production of virulence factors, reflected by a loss in hemolytic activity, without affecting bacterial growth. To our knowledge, these peptides represent the first potent synthetic inhibitors of quorum-sensing in B. cereus.
MeSH terms
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Amino Acid Sequence
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Amino Acid Substitution
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Animals
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Bacillus cereus / genetics*
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Bacillus cereus / metabolism
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Bacillus thuringiensis / genetics
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Bacterial Proteins / antagonists & inhibitors
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Bacterial Proteins / chemical synthesis
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Bacterial Proteins / chemistry
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Bacterial Proteins / pharmacology*
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Hemolysin Proteins / antagonists & inhibitors
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Humans
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Peptide Fragments / chemical synthesis
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Peptide Fragments / chemistry
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Peptide Fragments / pharmacology*
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Protein Engineering
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Quorum Sensing / drug effects*
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Regulon / drug effects*
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Sheep
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Trans-Activators / antagonists & inhibitors
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Virulence Factors / antagonists & inhibitors
Substances
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Bacterial Proteins
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Hemolysin Proteins
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PapR protein, Bacillus cereus
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Peptide Fragments
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PlcR protein, Bacillus
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Trans-Activators
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Virulence Factors