Annona muricata Leaf Extract Triggered Intrinsic Apoptotic Pathway to Attenuate Cancerous Features of Triple Negative Breast Cancer MDA-MB-231 Cells

Jee Young Kim; Thien T P Dao; Kwangho Song; Sait Byul Park; Hyeri Jang; Min Kyoung Park; Soo Ueng Gan; Yeong Shik Kim

doi:10.1155/2018/7972916

Annona muricata Leaf Extract Triggered Intrinsic Apoptotic Pathway to Attenuate Cancerous Features of Triple Negative Breast Cancer MDA-MB-231 Cells

Evid Based Complement Alternat Med. 2018 Jul 17:2018:7972916. doi: 10.1155/2018/7972916. eCollection 2018.

Authors

Jee Young Kim^{1

2}, Thien T P Dao¹, Kwangho Song¹, Sait Byul Park¹, Hyeri Jang¹, Min Kyoung Park¹, Soo Ueng Gan³, Yeong Shik Kim¹

Affiliations

¹ College of Pharmacy and Natural Product Research Institute, Seoul National University, Seoul 08826, Republic of Korea.
² Civil Appellate Division, Seoul Central District Court, Seoul 06594, Republic of Korea.
³ Westmoreland Alternative Medicine Association, 1801 Wilshire Boulevard, Los Angeles, CA 90057, USA.

Abstract

Annona muricata L., known as graviola, is an evergreen plant of the tropical regions and is a rich source of natural products. Graviola has various biological activities, and it is best known for its anticancer activity. This study aimed to investigate the effects of crude graviola extract in vitro on breast cancer cells; in particular, we aimed to identify an agent against triple negative breast cancer (TNBC). We used the TNBC MDA-MB-231 cell line as the experimental model and the ER(+) non-TNBC MCF-7 breast cancer cell line as the control. We identified annonaceous acetogenins, including annonacin isomers, characteristic to this plant by using liquid chromatography tandem mass spectrometry (LC/MS/MS). We observed a significant decrease in the cell viability in both cell lines within 48 h, whereas impaired cell motility and invasiveness were observed only in the MDA-MB-231 cell line. While the MCF-7 cells showed an ER-dependent mechanism of apoptosis, the apoptosis of MDA-MB-231 cells was governed by an intrinsic apoptotic pathway triggered by graviola leaf extract (GLE).