Approaching single DNA molecule detection with an ultrasensitive electrochemical genosensor based on gold nanoparticles and cobalt-porphyrin DNA conjugates

Chem Commun (Camb). 2018 Oct 2;54(79):11108-11111. doi: 10.1039/c8cc05362f.

Abstract

We describe an ultrasensitive electrochemical genosensor based on gold nanoparticles and cobalt-porphyrin functionalised ssDNA probes. The sensitivity at the attomolar concentration level arises from an increased density of redox labels on the electrode surface compared to sensors without NP modification. The electrode detects as few as 23 DNA molecules, approaching single molecule detection.

MeSH terms

  • Adsorption
  • Biosensing Techniques / methods*
  • Cobalt / chemistry
  • DNA, Single-Stranded / analysis*
  • DNA, Single-Stranded / chemistry*
  • DNA, Single-Stranded / genetics
  • Electrochemical Techniques / methods
  • Electrodes
  • Gold / chemistry*
  • Limit of Detection
  • Metal Nanoparticles / chemistry*
  • Metalloporphyrins / chemistry*
  • Nucleic Acid Hybridization
  • Oxidation-Reduction
  • Particle Size

Substances

  • DNA, Single-Stranded
  • Metalloporphyrins
  • Cobalt
  • Gold